96 pin multiblot replicator (AutoMate Scientific Inc)

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AutoMate Scientific Inc 96 pin multiblot replicator
96 Pin Multiblot Replicator, supplied by AutoMate Scientific Inc, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/96+pin+multiblot+replicator/MULTI-BLOT+REPLICATOR/10__1007_slash_s11557___013___0899___1-54-19-22
Average 94 stars, based on 15 article reviews

96 pin multiblot replicator - by Bioz Stars, 2026-10

94/100 stars

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Article Title: Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes
Article Snippet: Using a 96-pin multiblot replicator (V&P Scientific, Inc.), the master dilution plate was replica-plated in duplicate onto YPD plates containing no drug, 5 ng/mL rapamycin, 50 ng/mL rapamycin, 0.2 μg/mL cycloheximide, 2.0 μg/mL cycloheximide, 20 μg/mL anisomycin, 50 μg/mL anisomycin, 10 μg/mL hygromycin, or 100 μg/mL hygromycin.

Article Title: Role of ptsPorfTand sss Recombinase Genes in Root Colonization by Pseudomonas fluorescens Q8r1-96
Article Snippet: sssF , 5′ GTG CCC TTT GTC AGC CTG ATC CTG TTC G 3′.

Clone Assay:

Article Title: Role of ptsPorfTand sss Recombinase Genes in Root Colonization by Pseudomonas fluorescens Q8r1-96
Article Snippet: .. The library was arrayed on BrightStar-Plus nylon membranes (7.4 by 11.4 cm; Ambion, Inc., Austin, Tex.) by replicating clones from the glycerol stocks with a 96-pin multiblot replicator (V&P Scientific, Inc., San Diego, Calif.) and a library copier that permits arraying in a 386-sample format. ..

Article Title: Role of ptsP , orfT , and sss Recombinase Genes in Root Colonization by Pseudomonas fluorescens Q8r1-96
Article Snippet: The library was arrayed on BrightStar-Plus nylon membranes (7.4 by 11.4 cm; Ambion, Inc., Austin, Tex.) by replicating clones from the glycerol stocks with a 96-pin multiblot replicator (V&P Scientific, Inc., San Diego, Calif.) and a library copier that permits arraying in a 386-sample format. .. The library was arrayed on BrightStar-Plus nylon membranes (7.4 by 11.4 cm; Ambion, Inc., Austin, Tex.) by replicating clones from the glycerol stocks with a 96-pin multiblot replicator (V&P Scientific, Inc., San Diego, Calif.) and a library copier that permits arraying in a 386-sample format. .. The sss and orfT hybridization probes were amplified by PCR performed with Taq DNA polymerase (Promega, Madison, Wis.) and primers SSS_UP and SSS_LOW and primers ORFT_UP and ORFT_LOW (Table 2), respectively.

Polymerase Chain Reaction:

Article Title: Identification of fungal genes involved in the preinfection events between ectomycorrhizal association (Pisolithus tinctorius and Pinus massoniana)
Article Snippet: The preinfection stage of establishment of the mycorrhiza before physical contact between symbionts is crucial, as changes that occur throughout mycorrhiza formation are set in motion at this time.. To improve the understanding of the molecular mechanisms involved in the fungus Pisolithus tinctorius during its interaction with its symbiotic partner Pinus massoniana, we set up a cellophane-separated culture system avoiding physical contact between the symbionts.. A cDNA subtraction library representing the differentially expressed genes of P.tinctorius during the preinfection stage was constructed using suppression subtractive hybridization combined with mirror orientation selection (SSH/MOS).

Membrane:

Article Title: Identification of genes expressed during Drosophila melanogaster gastrulation by using subtractive hybridization.
Article Snippet: A subtractive hybridization approach was used to identify genes that are expressed at the beginning of gastrulation.. We used tester DNA complimentary to RNA (cDNA) prepared from stages 6–7 embryos (gastrula) and excess driver cDNA from stages 2–4 embryos (syncytial blastoderm) to generate a gastrula-subtracted cDNA library.. A reverse Northern blot procedure used to analyze 105 subtracted clones showed that 65% had a level of expression at least 2.5-fold higher in stages 6–7 versus stages 2–4 embryos.

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